HPLC is a kind of column chromatography having a wide range of uses in pharmaceuticals and analytical chemistry as an effective separation tool. The column, which acts as the stationary phase plays a key role in the separation of components in HPLC. Stationary phases are usually composed of polar or non-polar compounds as per column type. Either polar or non-polar columns are used to separate compounds depending on the nature of the compound to be analyzed.
The mobile phase is pumped into the system with the help of mechanical pumps and the sample is introduced into the mobile phase using the injector. Continue reading Theoretical Plates in HPLC